The Center will provide reagents, probes, viruses or genetically altered mice developed by the members of the Center upon request and free of charge. Requests for such materials can be made directly to the investigators, or to Ann Mayhew, Executive Assistant for the Center at amayhew@umn.edu.
| Plasmid Constructs |
|
|
| |
|
|
| Item |
Description |
MCBDA Contact |
| PRED-Tracer:GIRK1 |
Custom-derived plasmid |
Dr. Kevin Wickman; wickm002@umn.edu |
| PRED-Tracer:GIRK1-Flag |
Custom-derived plasmid |
Dr. Kevin Wickman; wickm002@umn.edu |
| PRED-Tracer:GIRK2A |
Custom-derived plasmid |
Dr. Kevin Wickman; wickm002@umn.edu |
| PRED-Tracer:GIRK2B |
Custom-derived plasmid |
Dr. Kevin Wickman; wickm002@umn.edu |
| PRED-Tracer:GIRK2C |
Custom-derived plasmid |
Dr. Kevin Wickman; wickm002@umn.edu |
| PRED-Tracer:GIRK3 |
Custom-derived plasmid |
Dr. Kevin Wickman; wickm002@umn.edu |
| KOR-lacZ transgenic construct |
A reporter KOR-lacZ for generating animal model of KOR promoter activity. The animals were not maintained after the research is completed, but the construct is available. |
Dr. Li-Na Wei; weixx009@umn.edu |
| Expression vectors for transcription factors |
Expression vectors for transcription factors such as NF-kB, histone deacetylases, retinoic acid receptors, c-Myc, Sp1. |
Dr. Li-Na Wei; weixx009@umn.edu |
| pASPMCA4 |
Expression vector for antisense to PMCA4. pCI-neo harboring cDNA encoding nucleotides 71-443 of PMCA4 in the antisense orientation - Usachev et al., (2006) J Neurosci 26:311-318 |
Dr. Stanley Thayer; sathayer@umn.edu |
| pUMA |
Expression vector for Mitochondrially-targeted GFP. The green fluorescent protein gene fused to the mitochondrial targeting sequence from subunit VIII of cytochrome C oxidase driven by RSV promoter - Baron et al.(2003) Brain Res 993:124-132 |
Dr. Stanley Thayer; sathayer@umn.edu |
| |
|
|
| pmtAEQ |
Expression vector for Mitochondrially-targeted aequorin. Apoaequorin gene fused to the mitochondrial targeting sequence from subunit VIII of cytochrome C oxidase in pMT2 – Padua et al. (1998) Am J Physiol C615-C622 |
Dr. Stanley Thayer; sathayer@umn.edu |
| HA-MOR |
Insert: Rat MOR. Vector: pcDNA3, pcDNAmp, pZeo, pRP4, pINDsp1. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| HA-DOR |
Insert: Mouse DOR. Vector: pcDNA3, pcDNAmp, pZeo, pRP4, pINDsp1. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| MORFLAG |
Insert: Mouse MOR. Vector: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| DORFLAG |
Insert: Mouse DOR. Vector: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| MORGFP |
Vector: pEGFPN1, pRK5. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| MORRFP |
Vector: pERFP, pRK5. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| MORYFP |
Vector: pEYFP. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| MORCFP |
Vector: pECFP. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| DORGFP |
Vector: pEGFPN1, pRK5. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| DORRFP |
Vector: pERFP, pRK5. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| DORYFP |
Vector: pEYFP. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| DORCFP |
Vector: pECFP. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| HA-MOR/DT |
Insert: Rat MOR with DOR carboxyl tail sequence. Vector: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| HA-DOR/MT |
Insert: Mouse DOR with MOR carboxyl tail sequence. Vector: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| HA-MORS375A |
Insert: HA-MOR with S375 mutation. Vector: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| HA-MORS363/375 |
Insert: HA-MOR with Ser363, Thr370 and Ser375 mutated to Ala. Vector: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| MORS196AEGFP |
Insert: MORGFP with S196A mutation. Vector: pEGFPN-1. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| MORS196ACSTAEGFP |
Insert: MORGFP with S196A, T327A and C330S mutations. Vector: pEGFPN-1. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| DOR-Rleu |
Insert: DOR with Renilla leuciferase fused to carboxyl terminus. Vector: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| DTS-Rleu |
Insert: DOR with all carboxyl tail Ser and Thr mutated to Ala fused with Renilla leuciferase. Vector: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| ß-Arr1-GFP2 |
Insert: ß-Arrestin 1 fused to the carboxyl terminus of GFP2. Vector: GFP2-C3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| ß-Arr2-GFP2 |
Insert: ß-Arrestin 2 fused to the carboxyl terminus of GFP2. Vector: GFP2-C3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| pHISi-4x40 |
Insert: Mouse MOR 40bp/sequence (-745~-706). Vector: pHISi. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| pLacZi-4x40 |
Insert: Mouse MOR 40bp/sequence (-745~-706). Vector: pLacZ. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| pL1.3K/747 |
Insert: MOR distal promoter. Vector: pGL3-basic. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| pL1.3K/738 |
Insert: MOR distal promoter. Vector: pGL3-basic. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| pL1.3K/731 |
Insert: MOR distal promoter. Vector: pGL3-basic. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| pc1-Sox18 |
Insert: Mammalian expression plasmid. Vector pcDNA1.1/amp |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| pc1-Sox6 |
Insert: Mammalian expression plasmid. Vector pcDNA1.1/amp |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| pc1-Sox21 |
Insert: Mammalian expression plasmid. Vector pcDNA1.1/amp |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| pcFLAG-PU.1 |
Insert: Mammalian expression plasmid. Vector pcDNA-5_UT-FLAG. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| pJ6-PU.1 |
Insert: Mammalian expression plasmid. Vector: pJ6. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| P+1 |
Insert: MOR proximal promoter/(-450 ~ +1). Vector: pGL3-basic. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| P29 |
Insert: MOR proximal promoter/(-450 ~ -29). Vector: pGL3-basic. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| P66 |
Insert: MOR proximal promoter/(-450 ~ -66). Vector: pGL3-basic. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| P90 |
Insert: MOR proximal promoter/(-450 ~ -90). Vector: pGL3-basic. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| P100 |
Insert: MOR proximal promoter/(-450 ~ -100). Vector: pGL3-basic. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| P121 |
Insert: MOR proximal promoter/(-450 ~ -121). Vector: pGL3-basic. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| P189 |
Insert: MOR proximal promoter/(-450 ~ -189). Vector: pGL3-basic. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| P220 |
Insert: MOR proximal promoter/(-450 ~ -220). Vector: pGL3-basic. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| P249 |
Insert: MOR proximal promoter/(-450 ~ -249). Vector: pGL3-basic. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| hMOR (pCMV4) |
Insert: Human mu-opioid receptor. Vector: pCMV4. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| SV1 (pCMV5) |
Insert: Splice variant1 of human mu-opioid receptor. Vector: pCMV5. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| SV2 (pCMV5) |
Insert: Splice variant 2 of human mu-opioid receptor. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| hMOR-GFP |
Insert: Human mu-opioid receptor-GFP. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| pNRSE |
Insert: Mouse MOR (-450 ~ +12). Vector: pGL3-basic. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| pmNRSE |
Insert: Mouse MOR (-450 ~ +12, mutated NRSE sequence). |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| pGLNRSP |
Insert: Mouse MOR (-4.7kb ~ +20). |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| uAUG (+) |
Insert: mMOR UTR (-301 ~ +1) +LUC. Vector: pGL3-Promoter. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| uAUG #1 |
Insert: mMOR UTR + LUC, #1 uAUG mutation. Vector: pGL3-Promoter. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| uAUG #2 |
Insert: mMOR UTR + LUC, #2 uAUG mutation. Vector: pGL3-Promoter. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| uAUG #3 |
Insert: mMOR UTR + LUC, #3 uAUG mutation. Vector: pGL3-Promoter. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| uAUG #2, 3 |
Insert: mMOR UTR + LUC, #2, 3 uAUG mutation. Vector: pGL3-Promoter. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| uAUG (-) |
Insert: mMOR UTR + LUC, All uAUGs mutation. Vector: pGL3-Promoter. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| uAUG #1 +S |
Insert: mMOR UTR + LUC. Codon stopped after #1 uAUG. Vector: pGL3-Promoter. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| uAUG #2 +S |
Insert: mMOR UTR + LUC. Codon stopped after #2 uAUG. Vector: pGL3-Promoter. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| uAUG #3 +S |
Insert: mMOR UTR + LUC. Codon stopped after #3 uAUG. Vector: pGL3-Promoter. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| uAUG #2, 3 +S |
Insert: mMOR UTR + LUC. Codon stopped after #2, 3 uAUG. Vector: pGL3-Promoter. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| uAUG (-) +S |
Insert: mMOR UTR + LUC. Codon stopped after all uAUGs. Vector: pGL3-Promoter. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| uUAG #1 M+S |
Insert: mMOR UTR + LUC, #1 uAUG mutation. Stop codon introduced. Vector: pGL3-Promoter. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| uUAG #2 M+S |
Insert: mMOR UTR + LUC, #2 uAUG mutation. Stop codon introduced. Vector: pGL3-Promoter. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| uUAG #3 M+S |
Insert: mMOR UTR + LUC, #3 uAUG mutation. Stop codon introduced. Vector: pGL3-Promoter. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| uUAG #2, 3 M+S |
Insert: mMOR UTR + LUC#2, 3 uAUG mutation. Stop codon introduced. Vector: pGL3-Promoter. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| uUAG (-) M+S |
Insert: mMOR UTR + LUC, All uAUGs mutation. Stop codon introduced. Vector: pGL3-Promoter. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| IMP |
Insert: mMOR UTR + LUC. #1 uAUG context improved. Vector: pGL3-Promoter. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| #2, 3_IMP |
Insert: mMOR UTR + LUC, #2, 3 uAUG mutation. #1 uAUG context improved. Vector: pGL3-Promoter. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| SP6-LUC |
Insert: SP6 + LUC. Vector: Topo-TA. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| SP6-uAUG #1 IFR |
Insert: mMOR uORF + LUC (in-frame), #2, 3 uAUGs mutation. Vector: Topo-TA. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| SP6-uAUG #2 IFR |
Insert: mMOR uORF + LUC (in-frame), #1, 3 uAUGs mutation. Vector: Topo-TA. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| SP6-uAUG #3 IFR |
Insert: mMOR uORF + LUC (in-frame), #1, 2 uAUGs mutation. Vector: Topo-TA. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| SP6-uAUG (-) IFR |
Insert: mMOR uORF + LUC (in-frame), all uAUGs mutation. Vector: Topo-TA. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| SP6-uAUG (+) IFR |
Insert: mMOR uORF + LUC (in-frame). Contains all uAUGs. Vector: Topo-TA. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| pmMUEG #1 |
Insert: mMOR UTR + mouse MOR coding region + EGFP. Vector: pEGFP-N1. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| pmMUEG #2 |
Insert: mMOR UTR + mouse MOR coding region + EGFP, #3 uAUG mutation. Vector: pEGFP. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| pmMUEG #3 |
Insert: mMOR UTR + mouse MOR coding region + EGFP, all uAUGs mutation. Vector: pEGFP. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Back to Top |
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| Animals |
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| |
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| Item |
Description |
MCBDA Contact |
| GIRK1 knockout mice |
|
Dr. Kevin Wickman; wickm002@umn.edu |
| GIRK3 knockout mice |
|
Dr. Kevin Wickman; wickm002@umn.edu |
| GIRK4 knockout mice |
|
Dr. Kevin Wickman; wickm002@umn.edu |
| MOR-KO |
Gene altered: MOR-1 deleted. Background: SVJ129xC57B6. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| MORKN196A |
Gene altered: MOR-1 with single a.a. mutation: S196A. Background: SVJ129xC57B6. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Back to Top |
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| Viral Constructs |
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| |
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|
| Item |
Description |
MCBDA Contact |
| AdRSVmtAEQ |
Recombinant adenovirus harboring the apoaequorin gene fused to the mitochondrial targeting sequence from subunit VIII of cytochrome C oxidase. Available stocks are currently limited. Baron et al.(2003) Brain Res 993:124-132 |
Dr. Stanley Thayer; sathayer@umn.edu |
| Adenovirus type 5: DORGFP |
|
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Adenovirus type 5: HA-DOR |
|
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Adenovirus type 5: MORGFP |
|
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Adenovirus type 5: HA-MOR |
|
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Adenovirus type 5: DORDTS |
HA-DOR with all Ser and Thr in carboxyl tail mutated to Ala. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Adenovirus type 5: MOR363/370/375 |
HA-MOR with Ser363, Thr370 and Ser375 mutated to Ala. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Adenovirus type 5: MORS196AGFP |
MORGFP with S196A mutation. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Adenovirus type 5: MORS196ACSTAGFP |
MORGFP with S196A, T327A and C330S mutations. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Adenovirus type 5: GFP |
|
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Adenovirus type 5: Gαi2-Leu |
Rat Gαi2 with C352L mutation. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Adenovirus type 5: Gαi3-Leu |
Rat Gαi3 with C351L mutation. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Adenovirus type 5: Gαo-Leu |
Rat Gαo with C351L mutation. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Back to Top |
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| Antibodies |
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| |
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| Item |
Description |
MCBDA Contact |
| Anti-GIRK2A/C |
Custom-derived antibody |
Dr. Kevin Wickman; wickm002@umn.edu |
| Anti-GIRK2C |
Custom-derived antibody |
Dr. Kevin Wickman; wickm002@umn.edu |
| Anti-GIRK3 |
Custom-derived antibody |
Dr. Kevin Wickman; wickm002@umn.edu |
| Anti-GIRK4 |
Custom-derived antibody |
Dr. Kevin Wickman; wickm002@umn.edu |
| Back to Top |
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| Cell Lines |
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| Item |
Description |
MCBDA Contact |
Mouse neuroblastoma x rat glioma NG10815 |
Gene transfected: None. Vector system: None. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Mouse neuorblastoma N18TG2 |
Gene transfected: None. Vector system: None. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Mouse neuroblastoma N1E115 |
Gene transfected: None. Vector system: None. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Mouse neuroblastoma NS20Y |
Gene transfected: None. Vector system: None. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Rat glioma C6 |
Gene transfected: None. Vector system: None. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Rat glioma C6BU1 |
Gene transfected: None. Vector system: None. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Rat pheochromocytoma PC12h |
Gene transfected: None. Vector system: None. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Human neuroblastom NMB |
Gene transfected: None. Vector system: None. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Human neuroblastoma SHSY5Y |
Gene transfected: None. Vector system: None. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Chinese Hamster Ovary DORBH32 |
Gene transfected: DOR. Vector system: pcDNA1neo. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Chinese Hamster Ovary DORX1-15 |
Gene transfected: DOR. Vector system: pcDNA1neo. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Chinese Hamster Ovary DORS177L |
Gene transfected: DORS177L. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Chinese Hamster Ovary HYP8-1 |
Gene transfected: HA-ORL-1. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Chinese Hamster Ovary KORS187L |
Gene transfected: KORS187L. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Chinese Hamster Ovary MOR |
Gene transfected: MOR. Vector system: pcDNAmp. |
|
| Dr. Ping-Yee Law; lawxx001@umn.edu |
| Chinese Hamster Ovary MORS196L |
Gene transfected: MORS196L. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Human Embryonic Kidney 293 HA-DOR |
Gene transfected: HA-DOR. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Human Embryonic Kidney 293 HA-DORT335A |
Gene transfected: HA-DORT335A. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Human Embryonic Kidney 293 HA-DORS344A |
Gene transfected: HA-DORS344A. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Human Embryonic Kidney 293 HA-DORT352A |
Gene transfected: HA-DORT352A. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Human Embryonic Kidney 293 HA-DORT353A |
Gene transfected: HA-DORT353A. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Human Embryonic Kidney 293 HA-DORT358A |
Gene transfected: HA-DORT358A. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Human Embryonic Kidney 293 HA-DORT361A |
Gene transfected: HA-DORT361A. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Human Embryonic Kidney 293 HA-DORS363A |
Gene transfected: HA-DORS363A. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Human Embryonic Kidney 293 HA-DORT361/S363A |
Gene transfected: HA-DORT361A and S363A. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Human Embryonic Kidney 293 HA-DTS |
Gene transfected: HA-DORDTS (all Ser and Thr in CT mutated to Ala). Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Human Embryonic Kidney 293 HA-DORT361D |
Gene transfected: HA-DORT361D. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Human Embryonic Kidney 293 HA-DORT363D |
Gene transfected: HA-DORS363D. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Human Embryonic Kidney 293 HA-DORCT/A363S |
Gene transfected: HA-DOR with all CT Ser/Thr mutated to Ala except Ser363. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Human Embryonic Kidney 293 HADORCT/361T/S363D |
Gene transfected: HA-DOR with all CT Ser/Thr mutated to Ala, except Thr361 and Ser363 are mutated to Asp. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Human Embryonic Kidney 293 DORGFP |
Gene transfected: DORGFP. Vector system: pEGFPN1. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Human Embryonic Kidney 293 HA-DORpINDsp1 |
Gene transfected: HA-DOR. Vector system: pINDsp1. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Human Embryonic Kidney 293 KORFLAG |
Gene transfected: rKORFLAG Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Human Embryonic Kidney 293 HA-MOR/DORFLAG |
Gene transfected: HA-MOR/DORFLAG. Vector system: pcDNAmp/pINDsp1. |
|
| Dr. Ping-Yee Law; lawxx001@umn.edu |
| Human Embryonic Kidney 293 HA-MOR |
Gene transfected: HA-MOR. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Human Embryonic Kidney 293 HA-MOR(354-357) |
Gene transfected: HA-MOR with Thr354, Ser355, Ser356 and Thr357 mutated to Ala. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Human Embryonic Kidney 293 HA-MOR(354-364) |
Gene transfected: HA-MOR with Thr354, Ser355, Ser356, Thr357, Ser363 and Thr364 mutated to Ala. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Human Embryonic Kidney 293 HA-MOR(354-376) |
Gene transfected: HA-MOR with Thr354, Ser355, Ser356, Thr357, Ser363, Thr364, Thr370, Ser375 and Thr376 mutated to Ala. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Human Embryonic Kidney 293 HA-MOR(354-394) |
Gene transfected: HA-MOR with Thr354, Ser355, Ser356, Thr357, Ser363, Thr364, Thr370, Ser375, Thr376,Thr379, Thr383 and Thr393 mutated to Ala. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Human Embryonic Kidney 293 HA-MOR(363-376) |
Gene transfected: HA-MOR with Ser363, Thr364, Thr370, Ser375 and Thr376 mutated to Ala. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Human Embryonic Kidney 293 HA-MOR(363/364) |
Gene transfected: HA-MOR with Ser363 and Thr364 mutated to Ala. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Human Embryonic Kidney 293 HA-MOR(375/376) |
Gene transfected: HA-MOR with Ser375 and Thr376 mutated to Ala. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Human Embryonic Kidney 293 HA-MOR363 |
Gene transfected: HA-MORS363A. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Human Embryonic Kidney 293 HA-MOR364 |
Gene transfected: HA-MORT364A. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Human Embryonic Kidney 293 HA-MOR370 |
Gene transfected: HA-MORT370A. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Human Embryonic Kidney 293 HA-MOR375 |
Gene transfected: HA-MORS375A. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Human Embryonic Kidney 293 HA-MOR376 |
Gene transfected: HA-MORT376A. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Human Embryonic Kidney 293 HA-MOR363/370/375 |
Gene transfected: HA-MOR with Ser363, Thr370 and Ser375 mutated to Ala. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Human Embryonic Kidney 293 HA-MOR370/375 |
Gene transfected: HA-MOR with Thr370 and Ser375 mutated to Ala. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Human Embryonic Kidney 293 HA-MOR363/375 |
Gene transfected: HA-MOR with Ser363 and Ser375 mutated to Ala. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Human Embryonic Kidney 293 HA-MOR363/370 |
Gene transfected: HA-MOR with Ser363 and Thr370 mutated to Ala. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Human Embryonic Kidney 293 MORGFP |
Gene transfected: MORGFP. Vector system: pEGFPN1. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Human Embryonic Kidney 293 MORS196L |
Gene transfected: MORS196L. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Human Embryonic Kidney 293MORS196LT327A |
Gene transfected: MOR with S196L and T327A mutations. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Human Embryonic Kidney 293 MORS196LC330S |
Gene transfected: MOR with S196L and C330S mutations. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Human Embryonic Kidney 293MORS196LCSTA |
Gene transfected: MOR with S196L, T327A and C330S mutations. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Human Embryonic Kidney 293 HA-MORi31 |
Gene transfected: HA-MOR with sequence Arg258 to Val262 deleted. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Human Embryonic Kidney 293 HA-MORi32 |
Gene transfected: HA-MOR with sequence Arg263 to Ser266 deleted. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Human Embryonic Kidney 293 HA-MORi33 |
Gene transfected: HA-MOR with sequence Gly267 to Lys271 deleted. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Human Embryonic Kidney 293 HA-MORi34 |
Gene transfected: HA-MOR with sequence Asp272 to Leu275 deleted. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Human Embryonic Kidney 293 HA-MORi35 |
Gene transfected: HA-MOR with sequence Arg276 to Arg280 deleted. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Human Embryonic Kidney 293 HA-MOR5A |
Gene transfected: HA-MOR with sequence Arg258 to Val262 replaced with 5 Ala. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Human Embryonic Kidney 293 HA-MORR276A |
Gene transfected: HA-MORR276A. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Human Embryonic Kidney 293 HA-MORR277A |
Gene transfected: HA-MORR277A. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Human Embryonic Kidney 293 HA-MORI278A |
Gene transfected: HA-MORI278A. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Human Embryonic Kidney 293 HA-MORT279A |
Gene transfected: HA-MORT279A. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Human Embryonic Kidney 293 HA-MORR280A |
Gene transfected: HA-MORR280A. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Human Embryonic Kidney 293 HA-MORR276A/R277A |
Gene transfected: HA-MOR with R276A and R277A mutations. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Human Embryonic Kidney 293 HA-MORC2 |
Gene transfected: HA-MOR with sequence Lys344 to Arg348 deleted. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Human Embryonic Kidney 293 MORFLAG/HA-DORΔi35 |
Gene transfected: MORFLAG; HA-DOR with Arg257 to Arg261 deleted. Vector system: pcDNAmp/pINDsp1. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Human Embryonic Kidney 293 HA-MOR |
Gene transfected: HA-MOR. Vector system: pINDsp1. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Neuroblastoma N2A DOR12-3 |
Gene transfected: HA-DOR. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Neuroblastoma N2A (His)6DOR |
Gene transfected: DOR with a (His)6 tag at the N-terminus. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Neuroblastoma N2A HYP8-1 |
Gene transfected: ORL-1. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Neuroblastoma N2A MORID2 |
Gene transfected: HA-MOR. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Neuroblastoma N2A (His)6MOR |
Gene transfected: MOR with a (His)6 tag at the N-terminus. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Neuroblastoma N2A HA-DOR/MT |
Gene transfected: HA-DOR with the carboxyl tail sequence of MOR. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Neuroblastoma N2A HA-MOR/DT |
Gene transfected: HA-MOR with carboxyl tail sequence of DOR. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Neuroblastoma N2A HA-MOR363D |
HA-MOR with sequence after Ser363 deleted. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Neuroblastoma N2A HA-DORL245M |
Gene transfected: HA-DOR with L245M mutation. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Neuroblastoma N2A HA-DOR/MTL245M |
Gene transfected: HA-DOR with the carboxyl sequence of MOR and L245M. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Neuroblastoma N2A HA-MORM264L |
Gene transfected: HA-MOR with M264L mutation. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
| Neuroblastoma N2A HA-MORE/DTM264L |
Gene transfected: HA-MOR with DOR carboxyl tail sequence and M246L mutation. Vector system: pcDNA3. |
Dr. Ping-Yee Law; lawxx001@umn.edu |
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| Item |
Description |
MCBDA Contact |
| microRNA (MiRna) microarray system |
This system is an essential experimental platform to investigate miRNA expression at a genome-wide scale. The probe set is designed based on the Sanger miRBase Sequence Database, Release 9.0 (October 2006). It contains 1,140 different 34-44 nt long oligonucleotides. They are complementary to C. elegans, Drosophila, zebrafish, mouse, rat, and human miRNAs, and also include a 28S rRNA probe, a human U6 snRNA probe, 10 human snoRNA probes, and 76 mismatch probes. |
Dr. Yan Zeng; zengx033@umn.edu |
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